Functional Properties of Human iPSC-Derived NGN2 Neurons

Functional Properties of Human iPSC-Derived NGN2 Neurons

News
14.10.2024

At Neuroservices-Alliance, we are excited to present our latest research findings on human iPSC-derived NGN2 neurons electrophysiology in collaboration with Biogen. Our poster, showcased at the SfN 2024, delves into the functional characterization of NGN2-derived neurons using both patch clamp and high-density microelectrode array (HD-MEA) techniques.

Why NGN2 Neurons?

Human iPSC-derived neurons, such as NGN2 neurons, offer a promising in vitro model to predict therapeutic efficacy for neuroscience research. While most studies focus on their mRNA expression, our research goes a step further by highlighting their electrophysiological properties. This approach allows for a more comprehensive understanding of neuronal function and drug responsiveness.

Key Findings

  • NGN2 neurons exhibited spontaneous action potential firing, with functional synapse formation indicated by the detection of spontaneous postsynaptic currents.
  • We observed network connectivity, characterized by coherent bursting patterns in NGN2 neurons using HD-MEA recordings. This network activity was modulated by the co-culture of NGN2 neurons with human iCellGABA inhibitory neurons.
  • Both adenosine and CX614 showed expected modulation of neuronal activity, validating NGN2 neurons as a reliable model for drug discovery programs.

Our work not only demonstrates the physiological relevance of NGN2 neurons but also underscores their potential for supporting lead optimization in drug development, especially for CNS therapies.

Download our poster below

Electrophysiological profiling the functional properties of human NGN2 neurons – Neuroscience 2024

*R. E. PETROSKI1, C. M. PETROSKI1, D. LIU1, S. GRIGORYEV1, M. HE1, K. LEE2, C. A. HINCKLEY2

1Neuroservices-Alliance, San Diego CA 92121, 2Biogen

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