# Neuroservices-Alliance

> Neuroservices-Alliance is a preclinical Contract Research Organization (CRO) focused
> exclusively on central nervous system (CNS) and pain drug discovery. Founded in 2006, it
> provides in vitro, ex vivo and in vivo electrophysiology services including patch clamp,
> multi-electrode array (MEA), high-density MEA, calcium imaging, acute brain and spinal cord
> slice recordings, in vivo spinal cord electrophysiology and dorsal root ganglion (DRG)
> recordings. Laboratories are located at Le Puy Sainte Réparade near Aix-en-Provence, France
> (headquarters), at the Neurocentre Magendie in Bordeaux, France, and in San Diego,
> California, USA. Recordings are performed on human iPSC-derived neurons from healthy donors
> and patients, on mouse and rat tissue (wild type and transgenic), and on DRG neurons from
> rodents, rabbit and non-human primates including cynomolgus, and on human brain slices.
> Clients are pharmaceutical and biotechnology companies worldwide.

## Expert Platforms

- [Cell Electrophysiology](https://www.neuroservices-alliance.com/experts-platform/cell-electrophysiology/): Patch clamp, MEA, HD-MEA and calcium imaging recordings from cloned channels and receptors, primary rodent neurons, DRG sensory neurons and human iPSC-derived neurons. Covers voltage-gated ion channels, ligand-gated ion channels and GPCRs.
- [Brain Slice Electrophysiology](https://www.neuroservices-alliance.com/experts-platform/brain-slice-electrophysiology/): Acute mouse, rat and human brain and spinal cord slice recordings, wild type or transgenic, using patch clamp, MEA and high-density MEA. Measures synaptic transmission, network activity and synaptic plasticity.
- [In vivo Brain Electrophysiology](https://www.neuroservices-alliance.com/experts-platform/in-vivo-brain-electrophysiology/): Single-unit and local field potential extracellular recordings plus EEG in behaving rodents across awake, slow wave sleep and REM states. Optogenetics coupled to in vivo electrophysiology for circuit-level target engagement.
- [In vivo Spinal Cord & DRG Electrophysiology](https://www.neuroservices-alliance.com/experts-platform/in-vivo-spinal-cord-electrophysiology/): Extracellular recordings from spinal cord dorsal horn neurons and dorsal root ganglia in deeply anesthetized rodents. Assesses nociceptor activity and analgesic efficacy on pain-specific endpoints, supporting non-opioid analgesic programs.

## Techniques

- [Patch-Clamp](https://www.neuroservices-alliance.com/patch-clamp/): Whole-cell, perforated-patch, cell-attached and outside-out configurations for single-neuron and single-channel resolution.
- [MEA (Multi-Electrode Array)](https://www.neuroservices-alliance.com/multi-electrode-array/): Parallel extracellular recording across a single brain or spinal cord slice, giving a macroscopic view of neuronal network activity.
- [HD-MEA (High-Density MEA)](https://www.neuroservices-alliance.com/high-density-mea/): MaxWell Biosystems MaxTwo system, over 26,000 electrodes per well across six parallel wells, resolving activity at sub-cellular resolution.
- [Calcium Imaging](https://www.neuroservices-alliance.com/calcium-imaging/): Fluorescent indicator-based imaging of intracellular calcium dynamics in individual neurons and neuronal networks.
- [All Techniques](https://www.neuroservices-alliance.com/our-techniques/): Overview of functional readouts available across platforms.

## Therapeutic Areas

- [Pain](https://www.neuroservices-alliance.com/therapeutic-area/neuropathic-pain/): Neuropathic and inflammatory pain, nociception, wind-up and central sensitization.
- [Epilepsy](https://www.neuroservices-alliance.com/therapeutic-area/epilepsy/)
- [Alzheimer's Disease](https://www.neuroservices-alliance.com/therapeutic-area/alzheimers/)
- [Parkinson's Disease](https://www.neuroservices-alliance.com/therapeutic-area/parkinson-disease/)
- [Huntington's Disease](https://www.neuroservices-alliance.com/therapeutic-area/huntington-disease/)
- [ALS](https://www.neuroservices-alliance.com/therapeutic-area/als/)
- [Schizophrenia](https://www.neuroservices-alliance.com/therapeutic-area/schizophrenia/)
- [Anxiety / Stress / PTSD](https://www.neuroservices-alliance.com/therapeutic-area/anxiety-stress/)
- [Drug addiction](https://www.neuroservices-alliance.com/therapeutic-area/drug-addiction/)
- [Autism spectrum disorder](https://www.neuroservices-alliance.com/therapeutic-area/autism-spectrum-disorder/)
- [ADHD](https://www.neuroservices-alliance.com/therapeutic-area/adhd/)
- [CNS Safety](https://www.neuroservices-alliance.com/therapeutic-area/cns-safety/)
- [All Therapeutic Areas](https://www.neuroservices-alliance.com/therapeutic-areas/)

## FAQs

- [FAQs](https://www.neuroservices-alliance.com/faqs/): Answers to common scientific questions about the platforms, models and readouts.
- [iPSC Cells for Drug Discovery: Assays, Pain Models, and CRO Evaluation](https://www.neuroservices-alliance.com/ipsc-cells-for-drug-discovery-assays-pain-models-and-cro-evaluation/): Which iPSC-based assays answer pain drug discovery questions, how to choose readouts, and what to look for in an iPSC-capable CRO.

## Scientific Blog Posts

- [Assaying Synaptic Plasticity in Brain Slices: Paired Patch-Clamp and MEA Recordings in Ketamine-Treated Rats](https://www.neuroservices-alliance.com/assaying-synaptic-plasticity-in-brain-slices-paired-patch-clamp-and-mea-recordings-in-ketamine-treated-rats/)
- [Cell Electrophysiology in Drug Discovery: Functional Assays and Endpoints Across Neuron Models](https://www.neuroservices-alliance.com/cell-electrophysiology-in-drug-discovery-functional-assays-and-endpoints-across-neuron-models/)
- [From Nature's Toxins to the Pages of Nature: Supporting Neurotoxin Research at Scripps](https://www.neuroservices-alliance.com/from-natures-toxins-to-the-pages-of-nature-supporting-neurotoxin-research-at-scripps/)
- [All Scientific Blog Posts](https://www.neuroservices-alliance.com/news/scientific-blog-posts/)

## Company

- [About Us](https://www.neuroservices-alliance.com/about-us/): Founded 2006. Laboratories in Le Puy Sainte Réparade and Bordeaux (France) and San Diego, California (USA).
- [The Team](https://www.neuroservices-alliance.com/the-team/): Scientific and management team.
- [On-Demand Resources](https://www.neuroservices-alliance.com/on-demand-resources/): Scientific posters and data samples.
- [Careers](https://www.neuroservices-alliance.com/careers-at-nsa/): Open positions — careers@neuroservices-alliance.com
- [Contact](https://www.neuroservices-alliance.com/contact/): contact@neuroservices-alliance.com